These ESTs were assembled in 296 contigs and 1092 singletons, CB-839 manufacturer resulting in 1388 unique sequences with a redundancy of 49.3% (Table 1). The majority of the contigs assembled ESTs from a maximum of four libraries, suggesting that these genes are expressed under environmental stress or specific growth conditions. The search results and GenBank submission numbers for each EST are shown in Additional file 1. Analysis of these 1388 unigenes revealed 666 sequences that had no similarity to the sequences in the GenBank dbEST, which contains 37890 T. rubrum sequences. Of the 666 sequences, 404 had no similarities to the sequences

in the nonredundant database (Table 1). Additional analysis revealed that of the 666 sequences, 91 were present https://www.selleckchem.com/products/netarsudil-ar-13324.html in the TrED database [16]. Thus, 575 novel genes were identified, representing a marked increase in the number of expressed genes JIB04 supplier identified in the dermatophyte T. rubrum. These genes and the corresponding libraries in which they were identified are highlighted in Additional file 2. Table 1 General features of T. rubrum EST

libraries Library GenBank accession No. No. of raw ESTs No. of contigs No. of singletons Unique genes No. of unigenes matching GenBank database (NR)(a) No. of unigenes without match to GenBank dbEST database(b)               matching GenBank database (NR) (c) without match to GenBank database (NR) Total FE524602-FE527336 2735 296 1092 1388 681 (49.1%) 262 (18.9%) 404 (29.1%) 1 FE524602-FE525578 977 75 545 620 235 (37.9%) 73 (11.8%) 207 (33.4%) 2 FE525579-FE525681 103 23 14 37 24 (64.9%) 18 (48.6%) 10 (27.0%) 3 FE525682-FE525782 101 7 76 83 46 (55.4%) 19 (22.9%) 20 (24.1%) 4 FE525783-FE526029 247 64 56 120 62 (51.7%) 31 (25.8%) 36 (30.0%) 5 FE526030-FE526148 119 7 50 57 26 (45.6%) 7 (12.3%) 17 (29.8%) 6 FE526149-FE526246 98 12 5 17 11 (64.7%) 5 (29.4%) 3 (17.6%) 7 FE526247-FE526554 308 36 59 95 69 (72.6%) 25 (26.3%) 17 (17.9%) 8 FE526555-FE526754 200 30 18 48 27 (56.3%) 21 (43.8%) 15 (31.3%) 9 FE526755-FE527126 and FG235008-FG235038 372 43 248 291 162 (55.7%) 53 (18.2%)

74 (25.4%) 10 FE527127-FE527336 210 26 143 169 106 (62.7%) 34 (20.1%) 23 (13.6%) (a) Unigenes with similarity to the sequences in the nonredundant NCBI database (1e-3) using BLASTx. (b) Unigenes with no similarity to the PIK3C2G sequences in the dbEST-NCBI database (1e-20) using BLASTn-Organism: Trichophyton rubrum (taxid:5551). (c) T. rubrum protein sequences identified in this database were excluded from this analysis. The 1388 unigenes identified in this study were functionally classified based on the Munich Information Center for Protein Sequences (MIPS) categories. The classification led to the identification of putative proteins involved in transcriptional regulation, cellular defense and stress, protein degradation, signaling, transport, and secretion, among other functions (Additional file 2). However, many of these unigenes (54.