ZM447439 was added to the MG132 treated cells blocked at the meiotic metaphase, many bivalents/chromosomes did not keep their position at the spindle equator. In more than 906 of MI spermatocytes and 64-character of MII spermatocytes, the bivalents/chromosomes were distributed through the entire cytoplasm. It is a statistically significant big difference compared to control cells treated with MG132 alone having all chromosomes in the spindle equator. In meiosis, the sister kinetochores are arranged side by side during MI thus ensuring the separation of maternal and paternal chromosomes while in MII the sister Pemirolast clinical trial kinetochores are arranged back to back ensuring chromatid segregation similar to mitosis. Our results showing that both MII and MI spermatocytes treated with ZM447439 fail to maintain metaphase chromosome alignment suggest that Aurora kinases regulate chromosome behavior independent of the sister kinetochore arrangement. We reviewed the spindle morphology in ZM447439 treated spermatocytes, because the observed chromosome alignment problems might be as a result of spindle failure. Research of tubulin stained spermatocytes unveiled that MG132 treated and metaphase caught cells had typical bipolar spindle morphology. But, a lot of the spermatocytes incubated in-the presence of MG132 plus ZM447439 displayed malformed spindles. The spindles were categorized into Mitochondrion five groups: bipolar prometaphase, bipolar metaphase, mono rod, multipole, and ribbon shaped spindles. Addition of ZM447439 to the spermatocytes pre treated with MG132 caused a significant increase in the number of cells with multiple spindle poles, 27-million of 29% MII extra spermatocyte and analyzed MI cells had more than one additional small microtubule organizing foci between the two main spindle poles. Furthermore, many primary and secondary spermatocytes demonstrated a ribbon shaped spindle in which most chromosomes were gathered into a single mass on the side of a bent spindle structure. Unlike MI, some secondary spermatocytes had chromosomes organized around a half spindle. These Ibrutinib molecular weight results suggest that Aurora kinases are required for the preservation of normal bipolar spindle morphology and metaphase chromosome alignment at MII and MI. During our research, we observed that many meiocytes handled with ZM447439 or microtubule drugs die. To investigate the mechanismof the observed cell death, we stained the trials for cleaved caspase 3, a marker for apoptotic cell death. We gathered level XIV tubule segments and cultured them in the presence of varied drugs up-to 2-4 h before sample planning, fixation, and staining for apoptotic cells. We observed a substantial increase in the range of cells positive for cleaved caspase 3 in the tubule segments incubated with ZM447439 for one day compared to controls cultured in the presence of DMSO.