We wish to thank C. Aubry for technical lab assistance and the Geneva Genomics Platform for qPCR assistance (Christelle Barraclough and Patrick Descombes). This work was supported by a Swiss National Foundation grant (PP00P3_128379), the NCCR Synapsy, the
Thorn Foundation, the Mercier Foundation, NARSAD (The brain and Behaviour Research Fund; A.D.) and by BIOSS Centre for Biological Signalling Studies (EXC 294, in support of L.H.). Abbreviations actb beta-actin actg1 gamma-actin adra1 alpha1 adrenergic (receptor) adra1d alpha1d adrenergic (receptor) adra2 alpha2 adrenergic (receptor) adra2a alpha2a adrenergic (receptor) adra2c alpha2c adrenergic (receptor) adrb beta adrenergic (receptor) adrb1 beta1 adrenergic (receptor) E embryonic day eef1a1 eukaryotic signaling pathway GSK2126458 manufacturer elongation factor-1 FACS fluorescence-activated cell sorting
Gusb beta-glucuronidase ko knockout PCR polymerase chain reaction SVZ subventricular zone VZ ventricular zone Figure S1. GAD65-GFP+ interneurons preferentially express a variety of markers expressed in CGE-derived interneurons but not MGE-derived interneurons. At P21 GAD65-GFP+ interneurons in the somatosensory cortex rarely express markers of cortical interneuron subtypes derived from the medial ganglionic eminences (MGE) such as somatostatin (A) or parvalbumin (B) but preferentially express markers of interneuron subtypes derived from the caudal ganglionic eminences (GGE) Orotidine 5′-phosphate decarboxylase such as VIP (C), reelin (D), NPY (E), calretinin
(F). Graph showing that at P21 in upper (I-IV) (G) and lower cortical layers (V-VI) (G’) of the somatosensory cortex GAD65-GFP+ interneurons express markers for CGE-derived interneurons (calretinin, VIP, NPY, reelin) and only rarely markers for MGE-derived interneurons (PV, SST). SST, somatostatin, PV, parvalbumin, VIP, vasoactive intestinal peptide, NPY, neuropeptide Y. Scale bar: 100 μm for A-B and 10 μm for C-F. Movie S1. Activation of adra1 with cirazoline affects interneuron migration. Time-lapse movie showing migrating GAD65-GFP positive cells under control conditions ascending from the intermediate zone towards the cortical plate (white arrows). After adra1 activation (cirazoline 500 mM; blue arrows) cells are halted in their migration. Movie S2. Activation of adra2 with medetomidine affects interneuron migration. Time-lapse movie showing migrating GAD65-GFP positive cells under control conditions ascending from the intermediate zone towards the cortical plate (white arrows). After adra2 activation (medetomidine 500 mM; blue arrows) cells are halted in their migration. Movie S3. Activation of adra2 with guanfacine affects interneuron migration. Time-lapse movie showing migrating GAD65-GFP positive cells under control conditions ascending from the intermediate zone towards the cortical plate (white arrows).