The luciferase activities were quantified by a Dual-Luciferase

Reporter Assay System (Promega), and the relative luciferase activity was calculated as the ratio of firefly to renilla luciferase activity, according to the manufacturer’s instructions. Each experiment was repeated three times. Statistical Analysis Statistical analysis was performed using the Chi-square test or analysis of variance (ANOVA) analysis for categorical variables and continuous variables, respectively. The Proc Allele procedure in the SAS/Genetics program (SAS Institute Inc., Cary, NC) was used to calculate linkage disequilibrium AG-881 cell line (LD). The Kaplan-Meier method and the log-rank test were used to estimate PFS and OS. The Cox proportional hazards regression model was used to analyze individual prognostic factors. All statistical tests were two-sided, a P value of 0.05 was considered statistically significant, and all analyses were performed using the Statistical Analysis System/Genetics software (SAS LY3039478 research buy version 9.13; SAS Institute Inc.) Results Demographic

and Blasticidin S manufacturer clinicopathologic characteristics of the study population have been described elsewhere [18]. Since there are significant racial differences in allele distributions of some SULF1 SNPs and the majority of the patients with available DNA samples were non-Hispanic whites (136/168, 80.9%), we included non-Hispanic whites only in further analysis. As shown in Table 2 of clinicopathologic characteristics in this study, the mean age of disease onset and standard deviation Glutamate dehydrogenase (SD) was 61.8 ± 10.7 years, and 12.5% were younger than 50 years. Among the 136 white patients, 91.9% had an advanced disease with 102 patients (75.6%) diagnosed at stage III and 22 patients (16.3%) diagnosed at stage IV. Most patients had high grade (127, 95.5%) and serous

cell type (109, 80.2%), and 85 patients (62.5%) had obtained optimal debulking during primary surgery. Table 2 Demographic and clinicopathologic characteristics in non-Hispanic white ovarian cancer patients Characteristics Number of patients % Age at Diagnosis (years) 136      <50 17 12.5    50 - 70 86 63.2    >70 33 24.3 Surgical stage a 135      I 5 3.7    II 6 4.4    III 102 75.6    IV 22 16.3 Tumor Grade a 133      1 6 4.5    3 127 95.5 Histology 136      Serous 109 80.2    Mucinous 2 1.5    Endometrioid 2 1.5    Clear cell 1 0.7    Brenner 3 2.2    Mixed 19 14.0 a Missing patient information: 1 for surgical stage; 3 for tumor grade Table 3 shows genotype distribution of the five SNPs. The LD analysis showed disequilibrium coefficient D’ = 0.965 and Correlation coefficient r 2 = 0.872 for rs6990375 G>A and rs3802278 G>A; D’ = 0.981 and r 2 = 0.678 for rs6990375 G>A and rs3087714 C>T; D’ = 1.000 and r 2 = 0.