The HOMA index was calculated after the dosage of insulin. Adiponectin level All biological samples were harvested in the morning before breakfast, and the serum was immediately separated by centrifugation and stored at -80°C until dosage was completed. This process was completed with recombinant human adiponectin by standard (Human Adiponectin RIA Linco Research® 6 research Park Dr St Charles, Missouri 63304 USA) using the instructions of manufacturer. Statistical analysis Statistical analysis was performed by Inhibitors,research,lifescience,medical using SPSS software (version 11, SPSS Inc, Chicago, IL, USA). Quantitative variables were expressed as median and range,

or as mean ± standard deviation when normally distributed. Parametric student’s test or non parametric Mann-Whitney’s test when appropriate were used to compare quantitative variables between the 2 groups. The relationship Inhibitors,research,lifescience,medical between the type of cancer and the other variables, especially the presence of diabetes and the rate of adiponectin was analyzed using χ2 test. A p value less than 0.05 was considered to indicate a significant difference. The threshold of adiponectin level was investigated by analysis of ROC curves and measuring the areas under the curves for a better sensitivity and specificity. For multivariate analysis, we used binary logistic regression Inhibitors,research,lifescience,medical to find the independent factors significantly associated with

adiponectin level (low or high comated with a threshold level of ADP) and diabetes with pancreatic Inhibitors,research,lifescience,medical cancer. The variables were analyzed in the multivariate model for a risk α < 10%. Values of p < 0.05 were considered statistically significant. Results ERK inhibitors high throughput screening Characteristics of patients Between January 2006

and September 2007, 53 consecutive patients with pancreatic adenocarcinoma and 30 with colorectal adenocarcinoma were analyzed. Mean age for Inhibitors,research,lifescience,medical the two groups was 69 years (range, 11.9 years). The mean HOMA index was 2.54 and the mean adiponectin level was 18.7 µg/L (range 2.9-74.5). The main demographic and clinical characteristics of all included patients are presented in Table 1. Table 2 shows the factors associated with the type of cancer. The two groups (pancreatic cancer and colorectal cancer) first were comparable for age, sex, BMI, the rate of cholestérol and tumour staging. Table 1 Characteristics of patients Table 2 Factors associated with the type of cancer, pancreatic vs colo-rectal (univariate analysis) In the pancreatic group there was however an increased incidence of hypertriglyceridemia (35.8% vs 9.1%, p = 0.05). Pancreatic cancer was associated with severe weight loss (BMI < 20) in 1/3 of the cases against 1/10 in the second group. At the moment of diagnosis, diabetes was two times more frequent in the group of patients with pancreatic cancer compared to patients presenting with colorectal cancer (39.6% vs 18.2%, p = 0.037).