Ould responsible for the activation S1P Receptors of ERK cord, JNK and p38 PI3K. In agreement, cytokines such as TNF and IL-1 which wrung mediation vinegar Acid reaction and the second phase of the formalin test induces neuronal and non neuronal activation of ERK, JNK, p38 and / or PI3K. Send foreigners A cytokine cascade stimulation PBQ Ger t of vinegar Acid, it induces the curved response h Depends on IL 18, IFN and endothelin-1. These mediators also induce ERK, JNK, p38 and / or activation of PI3K in neuronal cells and non-neuronal. Zun Were Highest two letters are used items in a reaction, to determine whether cause the difference in circumferential speed mediators in different spinal mechanisms would, and the formalin test was used because there are some similarities In relation to the peripheral mediators has Compared vinegar acid and demonstrated for the comparison with previous spinal mechanisms in the formalin test. However althoughaffinity ERA for BPA is very low, about 1/100 1/1000, that for estradiol 17b. On the other side of the estrogen receptor-gamma, a protein with a high binding affinity t for BPA is associated. However, the ERRC is not recognized as a receiver singer BPA, because constitutive transcriptional activity ERRC shows t without ligands. Here Including the investigation to the rapid modulation dopamine receptor signaling pathway of the line of nanomolar doses of BPA on the density and morphology of dendritic spines in adult hippocampal slices, Lich the study of receptors for BPA. To observe the direct effects of BPA on hippocampal neurons, we have isolated sweet Hippocampal slices, which have not projections of cholinergic neurons and serotonin from outside of the hippocampus. Second Materials and methods 2.1. Chemical BPA, 6 cyano 7 nitroquinoxaline-dione 2,3, cycloheximide, nicardipine, N-methyl-D-aspartate PD98059, ICI 182,780, MK 801 and Lucifer yellow were purchased from Sigma. 4 hydroxytamoxifen was obtained from Calbiochem. Other chemicals were obtained from h Chster purity commercially Ltlich. Polyclonal antibody Body ERRC was prepared by Dr. Shimohigashi Kyushu Univ .. 2.2. M adults Nnliche Animals Wistar rats were purchased from Saitama supply of laboratory animals. The experimental approach of this study was approved by the Committee on Animal Research of the University t of Tokyo. 2.3. Preparation of sweet hippocampal slices of rats were deeply With ethyl ether and decapitated sthesiert. The brains of adult rats were removed and placed at 4 ° C in artificial cerebrospinal fluid, consisting of: NaCl, 124 KCl, 5.0, 1.25 NaH2PO4, 2.0 MgSO 4, 2.0 CaCl 2, 22 NaHCO 3 , 10 glucose and was quilibriert with 95% O2 / 5% CO 2. The hippocampus was dissected and 300 lm thick transverse ridge resh To the L Longitudinal axis slices were prepared with a vibratome.sweet from these slices were prepared demonstration made Slices by incubation for 2 h rest at 25 ° C in ACSF. 2.4. Spinogenesis imaging and analysis of experimental details are described elsewhere. 2.4.1. Current injection of Lucifer Yellow sweet The discs were then incubated Vinorelbine with bisphenol A in the presence or absence of other drugs such as OH Tam or PD98059. Drug-treated discs were then upstream with 4% paraformaldehyde in PBS at 4 ° C for 2 4 hours. Neurons within slices were visualized by injection of Lucifer Yellow under a microscope Nikon E600FN.