On the contrary, a significant improvement in stability was noted with archaeosomes, which released only 20% during the same period. Figure 4 Release (%) of CF from Egg-PC/PEG45-Tetraether (90:10wt%) archaeosomes and from Egg-PC/PEG45-DSPE (90:10wt%) liposomes at (a) 4°C and (b) 37°C. Despite their apparent identical characteristics in terms of morphology and surface potential, PEGylated liposomes and archaeosomes Inhibitors,research,lifescience,medical exhibited different vesicle stabilities. The presence of
only 10wt% of archaeal tetraether lipid in the liposomal formulations increased significantly the nano-object stability and allowed a slow release of the encapsulated dye at 37°C. This enhanced stability could Inhibitors,research,lifescience,medical result from the membrane spanning organization of the PEGylated tetraether lipids within the Egg-PC bilayer membrane, forming a monolayer as previously shown with synthetic cationic tetraethers [13]. 4. Conclusions In conclusion, we have demonstrated that small proportions of a novel synthetic PEGylated archaeolipid added to a
liposomal formulation increase significantly the nanovector stability and slow down the constant dye release at 37°C. This result is quite promising in so far as a Fasudil in vitro similar behavior could be expected for in vivo applications. This study has also shown that HPTLC is a powerful method for analyzing lipid composition. Following Inhibitors,research,lifescience,medical such a fundamental work, we have recently evaluated the encapsulation of a therapeutic peptide (anticancer) extracted from marine resources into PEGylated archaeosomes and the in vivo efficiency of this peptide-loaded formulation. The first results are very promising and will be published Inhibitors,research,lifescience,medical elsewhere. Acknowledgments The authors would like to thank the partners of the project Sealacian for valuable Inhibitors,research,lifescience,medical discussion. They also would like to thank the CNRS, the Direction Générale des Entreprises (DGE), the Région Bretagne, and the Ministère de l’Enseignement Supérieur et de la Recherche
for financial support. Finally, the authors thank Dr. Olivier Lambert for cryo-TEM analysis. J. Barbeau would like to thank the DGE for the financial support, which enabled her to achieve this study.
The development of highly specific drug delivery systems (DDSs) holds a great promise for increased therapeutic treatment efficiency and elimination of often harmful side effects. Casein kinase 1 However, it is a formidable task due to additional strict requirements posed on DDS, such as high stability, ability to penetrate cellular membranes, and low cytotoxicity. Several important breakthroughs have been achieved in recent years using biologically inspired liposome, ligand, and antibody-based DDS, some of which are already used in clinical environment for cancer treatment with positive results [1, 2].