Of the 48 patients with positive pH/impedance findings, 38 was normal on WLI (Figure 1A) except 10 with EE and 37 showed positive AFI manifestations indicating the presence of GERD(Figure 1B). The sensitivity and accuracy of AFI (77% and 67%, respectively) in detecting GERD were higher than those of

WLI (20% and 52%, respectively), although the specificity of AFI (53%) was lower than that of WLI (97%). Inter-observer reliability analysis of AFI findings indicated substantial agreement (Kappa = 0.630, p = 0.000). Multivariate analysis showed that positive AFI findings significantly correlated with pH/impedance results (odds ratio [OR] = 0.242, 95% confidence interval [CI] 0.087–0.673, p = 0.007). Conclusion: AFI can detect esophageal PD0325901 solubility dmso mucosal ACP-196 mw changes related

to acid reflux, invisible on conventional WLI, in patients with NERD, suggesting that AFI endoscopy may be effective in the endoscopic diagnosis of GERD. Key Word(s): 1. Autofluorescence; 2. Endoscopy; 3. GERD; 4. Acid Reflux; Presenting Author: XI HUANG Additional Authors: JIUHONG MA Corresponding Author: XI HUANG Affiliations: The First Affiliated Hospital of Nanchang University Objective: Endoscopy is widely performed in China, but the reprocessing of the endoscope’s still poses many problems, because many units do not strictly adhere with to the endoscope reprocessing standard. To identify the practices used for reprocessing of gastrointestinal endoscopes in China, a survey a was carried out including basic information about the hospital, rinsing, disinfection, storage as well as personnel protection,

etc. Methods: A survey tool with 53 questions was designed for the survey, and 181 endoscope centers were investigated for endoscope reprocessing Oxymatrine by questionnaire. Results: The results indicate that the main method for the reprocessing of endoscope’s in the hospital endoscopy center’s was mainly manual processing (51.4%) with 45.8% of the endoscope centers using a combination of both manual reprocessing and Automated endoscope reprocessing. Only 2.8% of the investigated endoscope centers completely used Automated endoscope reprocessing. A lot of infection risks will emerge inevitably with such a high proportion of manual reprocessing procedures, which will be shown in the following survey results. 68.5% of the investigated hospitals chose glutaraldehyde, 10% chose the ortho-phthalaldehyde, 11.6% chose acidified water, 10% chose Chlorine dioxide and PAA. Conclusion: Although there are many endoscopy procedures carried out in China, there are still lots of issue’s during endoscope reprocessing which have not attracted extensive attention this study hope’s to address some of these issues. Key Word(s): 1. Reprocessing; 2. GI endoscopy; 3. Cleaning; 4.