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supports are shown by posterior probabilities from Bayesian inferences. Figure S3. SMART outputs representing the number of ANK motifs found in Pk1 translated sequences. Figure S4. SMART outputs representing the number of ANK motifs found in Pk2 translated sequences. Table S1. List of primers used in this study for sequencing (PCR), for expression analyses (RT-PCR), or for Southern blots (SB). Expected PCR product size in base pair (bp) was calculated relative to the wVulC reference sequences. BI 10773 Table S2. List of pk1 and pk2 sequences used for Figure 1, Additional file 1 : Figure S3 and Additional file 1 : Figure S4. Accession numbers from this study are in bold. (DOC 2 MB) References 1. Baldo L, Dunning Hotopp JC, Jolley KA, et al.: Multilocus sequence typing system for the endosymbiont Wolbachia pipientis. Appl Environ Microbiol 2006, 72:7098–7110.PubMedCrossRef 2. Bouchon D, Cordaux R, Grève P: Feminizing Wolbachia and the evolution of sex determination in isopods. In Insect Symbiosis. Edited by: Bourtzis K, Miller TA. Taylor & Francis Group, Boca Raton; 2008:273–294.CrossRef 3. Hilgenboecker K, Hammerstein P, Schlattmann P, Telschow A, Werren JH: How many species are infected with Wolbachia? A statistical analysis AG-881 in vitro of current data. FEMS Microbiol Lett 2008, 281:215–220.PubMedCrossRef 4.

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