(MJA 2009, 191: 232-234)”
“UV light induces DNA lesions, which are removed by nucleotide excision repair (NER). Exonuclease 1 (EXO1) is highly conserved from yeast to human and is implicated in numerous DNA metabolic Dibutyryl-cAMP in vivo pathways, including repair, recombination, replication, and telomere maintenance. Here we show that hEXO1 is involved in the cellular response to UV irradiation in human cells. After local UV irradiation, fluorescent-tagged hEXO1 localizes, together with NER factors, at the sites of damage in nonreplicating cells. hEXO1 accumulation requires XPF-dependent processing of UV-induced lesions and is enhanced by inhibition of DNA repair synthesis. In nonreplicating cells,
depletion of hEXO1 reduces unscheduled DNA synthesis after UV irradiation, prevents ubiquitylation of histone H2A, and impairs activation of the checkpoint signal transduction cascade in response to UV damage. These findings reveal a key role for hEXO1 in the UV-induced DNA damage response linking NER to checkpoint activation in human cells.”
“Diabetes mellitus (DM) is a growing health concern worldwide. Use of plants for treatment of DM is widely practiced in the Middle East. Garlic (Allium sativum L.) and Capparis spinosa are traditionally used as antidiabetic medications.
The present study evaluated the differential effects of ethanol extraction of these plants on the blood glucose concentration and the pathology of pancreatic beta-cell mass, liver, lungs, kidneys and GI tract in diabetic rats. DM was induced in 30 out Ruboxistaurin of 40 adult female P005091 research buy Albino rats, using intraperitoneal injection of 120 mg/kg BW alloxan. The diabetic rats were assigned into three groups, two of which were treated with extract of garlic and C. spinosa fruits (300 mg/kg) for 12 days and the rats of the third group, as the untreated group received ordinary diet. Ten non diabetic rats remained as the normal control group. Administration of these extracts tended to decrease the blood glucose concentration, while the blood glucose
of the untreated rats remained significantly high. The garlic extract was found to be more effective than C. spinosa extract. Histopathologically, tissue sections of the pancreas in the treated rats did not show a significant difference with the untreated diabetic rats. The liver of the treated diabetic rats with garlic extract revealed slight improvement in the hepatic tissue compared to those of the untreated diabetic rats. This study indicated a significant antihyperglycemic effect of garlic and C. spinosa fruit and supported its traditional usage in treatment of diabetes mellitus.”
“The acetyl benzo[f]coumarin condensed with phenyl hydrazine to afford the corresponding phenyl hydrazone which cyclized into the pyrazolyl benzocoumarin under Vilsmeier reaction conditions.