St 5 min incubation. Only 75.3 HA-1077 Fasudil and 46.6% of GA remained after 5 min of incubation and 120th Consistently came to the reduced GSH Born into a mini-site training in GAH2 HLM, and formed 85.7% of GAH2 1 min after the addition of 5 mM GSH reduced. Furthermore, the formation of GAH2 also may need during the incubation of GA and GSH in the absence of social housing has been observed, and the ratio Ratio of Peakfl Surface was GAH2 GA and 1.93 after 2 h of incubation. No significant amount of glutathione conjugates were detected in all incubations up with negative-ion MRM Trnsfer Length detected m / z 864 3 866 3 821 and 823rd In contrast to GA, 17 was AAG comprising Claim 1 mg / ml metabolized HLM and YOUR BIDDING disappeared 45 min after the start of incubation. Less than 1% of 17 was AAGH2 need during the first 30 minutes of incubation in HLM detected, and 17 AAGH2 was recorded after incubation for 1 h. The presence of reduced GSH not significantly Change the metabolism in HLM 17 AAG, but then causes a rapid onset born 17 AAGH2 training. In addition, 3.04% was obtained of 17 AAGH2 when 5 T 17 AAG was incubated with 5 mM reduced GSH for 2 h in the absence of HLM. Glutathione conjugates of 17-AAG did not need during the incubations with negative-ion MRM Trnsfer Length detected m / z 889 3 891 3 846 and 848th 2C shows the relative proportions of 17 DMAG and its metabolites may need during the incubation. Characterization of metabolites M1 to M7 17 DMAG is in the n Next section discussed. Compared with 17 AAG, DMAG exhibited improved metabolic stability of 17 t. Twenty-four percent of the 17 DMAG was in any public housing after 2 h of incubation. Only a trace amount of 17 DMAGH2 was obtained after incubation for 2 h.
Reduced GSH had no effect on the metabolism AMN-107 of 17 and 17 DMAG DMAGH2 formation in HLM. The maximum percentage of 17 DMAG SG may need during the incubation was detected at 60 min, w While glutathionyl 19 17 17 demethoxygeldanamycin hydroquinone was w Not detected during the incubation. In addition, 17-DMAG was obtained 1.16% SG, w Incubated during 17 DMAG with reduced GSH for 2 h in the absence of CPB. Identification of metabolites of 17 DMAG. For the fragment ions of protonated gr 17 th DMAG, the MS / MS and MS 3 spectra of GA, 17 AAG, DMAG and 17 shall be awarded. 3A shows the MS / MS spectrum of protonated 17 DMAG. Product ions at m / z 58 and 72 of 17 DMAG, which were not in the MS / MS spectra of AG and AAG 17 demonstrated dimethylethanamine groups were N, N, and the 17 assigned to trimethylamine cha dimethylaminoethylamino side do. Product ions at m / z 187 and 159 were detected in MS / MS spectra of the GA, 17 AAG and 17DMAG. Based on the accurate measurement of the mass, the product ion at m / z 187 to 10 was assigned to C1, as shown in FIG. 3B. MS3 spectra on the ion at m / z 187 was further increased to produce ions at m / z 159, 131 and 117, and their structures were tentatively proposed in Fig. 3B. In addition to the 17 and 17 DMAGH2 SG DMAG, 17-DMAG 12 metabolites were detected in the HLM incubation, but not in control Negative by full-scan MS and MRM scan. Three metabolites were having a protonated molecular ion of 665, five had a Mr of 633, we had a Mr of 627, we had a Mr of 615, and two had a gentleman from 603rd The main metabolites are the compounds with an M.