Effect of low concentrations

of dissolved oxygen on zoospore survival As in the dissolved oxygen elevation assays, the greatest colony counts in the control bottles occurred at 10-min exposure for P. megasperma and at 2- or 4-h exposure for the other three species (Table 3). Table 3 Linear regression analyses of colony counts (y) and levels (x) of dissolved oxygen reduction from that in the control Hoagland’s solution by Phytophthora species and exposure time z Species Exposure (h) Intercept ( a ) Slope ( b ) P P. megasperma 0 (10 min) 18.2 -1.0 0.0936   2 11.3 -0.2 0.6267   4 9.9 -0.8 0.0104   8 7.4 -0.3 0.2903   24 8.4 -0.7 0.0292   48 7.6 -0.9 0.0015   72 4.5 -0.3 0.0724 P. nicotianae 0 7.8 0.8 0.1067   2 25.0 -1.2 0.0548   4 28.5 -2.6 0.0008   8 12.3 -0.4 0.4421   24 5.1 -0.2 0.4100   48 3.6 0.0 0.8670

  72 2.2 0.1 0.3973 P. pini #https://www.selleckchem.com/products/crt0066101.html randurls[1|1|,|CHEM1|]# 0 9.1 0.4 0.2462   2 32.6 -0.3 0.6893   4 37.2 -2.1 0.0002   8 20.8 -1.3 < 0.0001 H 89 mouse   24 14.4 -0.8 0.0034   48 7.4 -0.3 0.2382   72 8.3 -0.5 0.0313 P. tropicalis 0 27.8 -1.8 0.0156   2 31.4 -1.3 0.0749   4 29.7 -0.3 0.6712   8 22.5 -0.1 0.8042   24 7.8 -0.3 0.1730   48 0.7 0.4 0.0008   72 0.4 0.2 0.0079 zLinear model: y = a + bx, in which x = 5.3 - meter readings of dissolved oxygen in the Hoagland’s solutions after being bubbled with pure nitrogen, so 0 ≤ x ≤ 5.3 mg L-1. Zoospore survival of the four species assessed in this study also was negatively impacted by low concentrations of dissolved oxygen in two distinct patterns (Table 3). One pattern is represented by P. megasperma and P. pini. The impact on these two species generally occurred at 4-h or longer exposures at which their colony counts decreased with increasing level of dissolved oxygen reduction from the normal concentration of 5.3 mg L-1 in the control Hoagland’s solution. The greatest rate of decrease in colony counts

occurred at 48-h exposure for P. megasperma at 0.9 colony per unit of dissolved oxygen reduction (P = 0.0015) and at 4-h exposure for P. pini at 2.1 (P = 0.0002). Phytophthora Succinyl-CoA nicotianae and P. tropicalis showed an exactly opposite pattern. The colony counts decreased with increasing level of reduction in dissolved oxygen concentration at both 2- and 4-h exposures for P. nicotianae, 10-min and 2-h exposures for P. tropicalis. These results indicate that P. nicotianae and P. tropicalis are more prone than P. megasperma and P. pini to hypoxia stress in aquatic environments. They help understand the more consistent and greater recoveries of P. megasperma and P. pini than other major plant pathogens including P. nicotianae and P.