The feasible energetic sites had been predicted by homologous modeling and molecular docking. By mutagenisis and catalytic task detection, three key energetic web sites, Glu391, His15, and Thr141, were identified, while Phe146 had been related to device variety. To sum up, we found that the lignan glycosyltransferase UGT236 from I.indigotica could catalyze the result of phloretin into phloridzin. Several key amino acid residues had been identified by framework prediction, molecular docking, and site-mutagenesis, which offered a basis for learning the specificity and variety of phloretin glycoside items. This study can provide a reference for artificially creating glycosyltransferase elements with a high effectiveness and certain catalysis.CRISPR-Cas9 gene editing technology has been widely used in Saccharomyces cerevisiae.However, the consequences of Cas9, as an exogenous protein, on the growth and creation of organic products in S.cerevisiae are still unclear.In this research, Cas9 gene had been expressed in S.cerevisiae by integration into the genome and building into vectors, and two organic products, carotenoid and miltiradiene, were selected as the target items to examine the effects of Cas9 expression on fungus development and production capacity.The results showed that whether Cas9 was integrated into the genome or expressed by vectors, Cas9 inhibited the development of S.cerevisiae, which was more apparent in the type of genome integration.When Cas9 ended up being integrated into the genome, it had no impact on manufacturing of carotenoid and miltiradiene by S.cerevisiae, nevertheless when Cas9 had been expressed by vectors, the power of S.cerevisiae to produce carotenoids and miltiradiene had been somewhat reduced.Therefore, so as to help expand efficiently knock aside Cas9 after gene modifying and lessen the unpleasant impact of Ura3 and Trp1 vectors, this study methodically explored the removal efficiency regarding the two vectors, and a plasmid with the capacity of efficient gene editing had been constructed, which optimized the application of CRISPR-Cas9 gene editing system in S.cerevisiae, and provided research for the application of gene modifying technology predicated on Cas9.The content of total flavonol glycosides in Ginkgo Folium when you look at the planting bases was based on high end liquid chromatography(HPLC).The samples had been extracted by reflux with methanol-25% hydrochloric acid.The HPLC conditions were as follows Agilent ZORBAX SB-C_(18) column(4.6 mm×250 mm, 5 μm), isocratic elution with cellular phase of 0.4% phosphoric acid solution-methanol(45∶55), circulation rate of 1 mL·min~(-1), column heat this website of 30 ℃, detection wavelength of 360 nm, and injection vo-lume of 10 μL.A method for the determination of terpene lactones in Ginkgo Folium had been founded according to ultra-high overall performance liquid chromatograph-triple-quadrupole/linear ion-trap combination mass spectrometry(UPLC-QTRAP-MS/MS).The UPLC circumstances were because below gradient elution with acetonitrile-0.1% formic acid, circulation price of 0.2 mL·min~(-1), line temperature of 30 ℃, test chamber temperature of 10 ℃, and shot level of 10 μL.The ESI~+and numerous response monitoring(MRM) had been used for the MS.The above techniques were used to determine the content of complete flavonol glycosides and terpene lactones in 99 batches of Ginkgo Folium from 6 sowing bases, additionally the results were statistically analyzed.The content of flavonoids and terpene lactones in Ginkgo Folium from various beginnings, from woods of various ages, gathered at different time, from woods of different genders, and processed with different ways was compared.The results indicated that the content of total flavonol glucosides in 99 Ginkgo Folium examples ranged from 0.38% to 2.08per cent, plus the total content for the four terpene lactones was in the number of 0.03%-0.87%.The technique established in this study is simple and trustworthy, that can easily be employed for the quantitative evaluation of Ginkgo Folium.The analysis outcomes lay a basis when it comes to quality-control of Ginkgo Folium.Light could be the primary source for plants to obtain energy.Asarum forbesii is an average tone medicinal plant, which typically Molecular Biology develops in the questionable and wet location underneath the bushes or beside the ditches.It can grow and develop without an excessive amount of light intensity.This test explores the results of shading from the growth, physiological attributes and power kcalorie burning of A.forbesii, that may supply reference and guidance for the synthetic planting.In this test, A.forbesii was planted under 80%, 60%, 40%, 20% and no tone.During the energetic growth period greenhouse bio-test , the photosynthetic physiological attributes such as for instance fluorescence parameters, photosynthetic variables, photosynthetic pigment content and ultrastructure, as well as the content of mitochondrial electron transport chain(ETC) synthase and nutrients were measured.The results revealed that the photosynthetic pigment content, chlorophyll fluorescence parameters and net photosynthesis rate(P_n) decreased using the decrease of shading.Under 20%-40% shading treatment, the plants had damaged ultrastructure, expanded and disintegrated chloroplast, disordered stroma lamella and grana lamella, and enhanced osmiophi-lic granules and starch granules.The tasks of nicotinamide adenine dinucleotide dehydrogenase(NADH), succinate dehydrogenase(SDH), cytochrome C oxidoreductase(CCO) and adenosine triphosphate(ATP) synthasewere favorably linked to light intensity.With the reduced amount of shading, this content of complete sugar and necessary protein in nutritional elements increased first and then decreased, additionally the content was the best under 60% tone.In conclusion, under 60%-80% shading therapy, the chloroplast and mitochondria had more complete structure, faster energy metabolic process, greater light energy-conversion efficiency, better absorption and utilization of light power and much more nutrient synthesis, that was considerably better for the growth and growth of A.forbesii.Attapulgite(ATP), as a fertilizer slow-release broker and earth conditioner, has shown remarkable impact in enhancing the usage rate of fertilizer therefore the yield and high quality of agricultural items and Chinese medicinal materials.