Briefly, a Waters large efficiency liquid chromatography process equipped that has a 2695 solvent delivery module and a 996 photodiode UV detector was utilised. The chromatographic separation of your analytes was attained by an Agilent Eclipse XDB C18 column linked to an Agilent C18 guard column. The mobile phase consisting of 0. 5% acetic acid in acetonitrile and 0. 5% acetic acid in water was run with gradient elution at a movement price of one mL min. The linear gradient elution was carried out as follows, solvent A was kept at 5% for the first five min and elevated to 10%, 17%, 35% and 90% in the upcoming 13 min, 12 min, 10 min and three min respectively, it had been then returned to 5% in five min and equilibrated for 15 min just before the subsequent injection.
HPLC evaluation indicated the selleck DG extract contained the fol lowing marker compounds, danshensu, salvianolic acid B, protocatechuic aldehyde, puerarin, daidzein eight C apiosyl glucoside, daidzin and daidzein. Pharmacokinetics research indicated that only danshensu, puerarin and daidzein have been detectable in plasma at thirty min immediately after oral administration of DG extract to rats at a dose of 0. 15 g kg. Animals Adult female Sprague Dawley rats have been housed in an air humidity managed area with 12 hour dark light cycle at approximately 22 C and permitted foods and water ad libitum inside the Animal and Plant Care Facility on the Hong Kong University of Science and Technologies during the experiments. All experimental procedures have been approved from the Analysis Practice Committee on the HKUST.
Induction of acute myocardial damage Animals have been randomly assigned to different groups of 6 animals in just about every for your induction of myocardial damage with or devoid of publish treatment with the DG extract. Animals received an intraperitoneal injec tion of ISO at a single dose of 200 mg kg for your induction myocardial injury. Pre liminary scientific studies indicated that the ISO knowing it administration improved plasma enzyme pursuits inside of six hours within the rats. Control animals obtained the motor vehicle only. Blood samples have been obtained from phenobarbital anesthetized rats at rising time intervals post ISO administration. These rats were then sacrificed by cardiac excision. Myocardial ventricular tissue samples had been obtained for the preparation of cytosolic and mitochondrial fractions for biochemical analyses.
Basal

values of plasma enzyme routines and myocardial mitochondrial parameters were obtained from animals sacrificed right away after the injection of saline. DG submit therapy protocol Animals have been intragastrically administered with all the DG extract at a dose of 4 g kg without delay following intraperito neal injection of ISO during the rat model of ISO induced acute myocardial injury. Preliminary research indicated that oral administration of the DG extract at 2 g kg did not generate any detectable adjustments in plasma enzyme pursuits four hrs soon after intraperitoneal injection of ISO in rats.