JAK2 rearrangements in some F Cases of AML seen atypical CML and ALL, w While exon 12 mutations in PV.3, 4,31,32 testing and detection of JAK2 mutations quantification determination of hetero-or homozygous allele and the load or reported in the report BMS 777607 of the mutant allele with the wild type, the Objectives of the molecular studies. Screening techniques variables are available and these are substantially identical to a sequential lacing direct allele-specific analysis of the individual in response to no polymerase or highly sensitive PCR assays with sensitivities variables that can recognize the mutated gene level of 20%, 3% and 0, 01% amount. However quantification techniques are preferred, the mutant allele to protect load beautiful to monitor progression of the disease and the effect of therapy. Peripheral blood samples and bone marrow plasma frozen and paraffin-embedded bone marrow biopsies Trphine k Can all be used.2, studied 33 Eric Lippert and his colleagues, the concordance Tofacitinib analysis con Ues for the quantification of JAK2 V617F and recorded gr Ere sensitivity using Taqman PCR with allele-specific primer amount to front and rear with a detection sensitivity of 0.2% and 0.15%. But they found this technique worked with false positive results and were as expected. Only able to detect the mutation of interest On the other hand, they found pyrosquen lacing lacing and sequential direct DNA as less sensitive, limited to 2% and 5%, but still have the advantage of detecting new mutations.34 was suggested recently that the Immunpr zipitation And Western blot test SOCS3 tyrosine phosphorylation of a new biomarker for the MPN and JAK2 mutation may be due to a journalist m Possible therapy potent inhibitor currently in clinical development. 26 The JAK2 his r Ment abnormalities of JAK2 at h Dermatological malignancies Best Klonalit t The long list of causes of congenital or secondary R reactive cytosis is cytopenia and dysplasia difficulties when creating the labeling conditions neoplastic without a process of extensive Ermittlungsma did not take the time ben CONFIRMS to persistence best term exp hnen requires. Best Term Klonalit Can handle all these problems and t k Can problematic F Lle in which a neoplastic condition with congenital or secondary Re Cause Co to existing l Sen. Many methods are used to detect Klonalit t, but the most reliable Ssigste is cytogenetics, fluorescence in situ hybridization to perform and / or molecular tests. Connected Immunph Genotype and abnormal loss of polymorphism X have many Restrict ONS In MPN. It is now generally accepted that the detection of JAK2 V617F mutation in particular is a major criterion in the diagnosis of tumors myelo Of particular MPNS. It is now in the 2008 edition of the WHO, 2007 and 2007 Nordic BCSH classifications.3, 35,36 and abnormal Ph Genotype determination JAK2 line are integrated into many types of h Dermatological malignancies mentioned above Seen reconciled, and are also in the 70 80% of ungekl rten hepatic vein thrombosis without overt neoplasms.37 Not only single V617F mutation is seen in a wide range of tumors myelo of.