Biotechniques 1999, 26:824–826. 828PubMed 36. Hoang TT, Karkhoff-Schweizer RR, Kutchma AJ, Schweizer HP: A broad-host-range Flp-FRT selleck products recombination system for site-specific excision

of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 1998, 212:77–86.CrossRefPubMed 37. Stachel SE, An G, Flores C, Nester EW: A Tn 3 lacZ transposon for the random generation of b -galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium. Embo J 1985, 4:891–898.PubMed 38. Irizarry RA, Hobbs B, Collin F, Beazer-Barclay YD, Antonellis KJ, Scherf U, Speed TP: Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics find more 2003, 4:249–264.CrossRefPubMed 39. Abramoff MD, Magelhaes PJ, Ram SJ: Image processing

with ImageJ. Biophotonics International 2004, 11:36–42. Authors’ contributions SS carried out all the experimental studies and participated in experimental design and drafting the manuscript. VV designed, coordinated the study and drafted the manuscript. Both selleck chemical authors read and approved the final manuscript.”
“Background Variovorax paradoxus is a ubiquitous, aerobic, gram negative bacterium present in diverse environments [1, 2]. This organism, originally classified in either the genus Alcaligenes or Hydrogenomonas, has been associated with a number of interesting biotransformations, including atrazine degradation [3], nitrotyrosine assimilation [4], and mineralization of acyl-homoserine lactone signals [5]. Recently, the hydrogen gas oxidation growth strategy of V. paradoxus has been implicated in plant growth promotion [6], as part of the rhizosphere consortium with nodulating diazotrophs. This microorganism was also recently identified as a member of methylotrophic community in the human oral cavity [7]. In spite of its ubiquity, and a wealth of interesting metabolic capacities, relatively little has been published on the physiology of V. paradoxus. The

morphology of bacterial colonies is an often described feature used in identification of isolates from diverse sources. It is frequently observed that colony morphology is a Edoxaban crucial indicator of strain variation [8], which has been used productively at least since Griffith’s experiments with pneumococci. Organisms such as Myxococcus xanthus have been studied extensively and productively to understand differentiation processes on a surface[9]. Gliding, swarming, swimming, and twitching motility have been categorized and catalogued in many species [10]. More recently, it has become clear that the complex communities of bacteria forming a colony on an agar plate can be used as a model system for studying growth physiology.