FC levels inparison to non-vaccinated control mice . The IL SFC levels were not  Asarylaldehyde statistically different from splenocytes stimulated with scrambled peptide . 5 Downloaded from clincancerres.aacrjournals on March 9, Copyright American Association for Cancer Research Author Manuscript Published OnlineFirst on March 0.  Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. We thenpared serum cytokine profiles following 4 hrs versus hrs after vaccination for the purpose of monitoring the polarization of cytokine response to T or T . Only 4 hr time points showed a T response suggesting that serum cytokine profiles are not sufficient for monitoring the immune response hrs post-vaccination.

Howev the ELISpot assay on splenocytes hrs post-vaccination showed highly significant  Silibinin 22888-70-6 IFN SFC levels for all vaccinated mice . Inparis the IL SFC levels were not significantly different from those of media/scrambled peptide stimulated splenocytes . These results confirm the T polarization is retained hrs post-vaccination. The difference between Luminex and ELISpot is probably due to the delayed nature of splenocytes vs. transient plasma cytokine changes. In a subset of 2 tumor bearing mi tamoxifen 0 mg/ letrozole mg/ and estradiol mg/kg were examined for antitumor activity. Survival waspared between vaccine treatment alone with antiestrogen/AI treated and vaccinated treatment groups. The tumor progression rates for vaccinated mice treated with tamoxifen or letrozole were not statistically different from the vaccine alone . Mice which were vaccinated and treated with estradiol had significantly shorter survivalpared to vaccine treatment alone . Letrozole and Tamoxifen inbination with L-BL .

The main aim of these L-BL 5bination studies was to determine whetherbining L-BL 5 with  buy Neohesperidin hormonal therapy provides any overall survival advantage over L-BL 5 or hormonal therapy alone. These studies were designed to use untreated and CTX placebo groups 6 Downloaded from clincancerres.aacrjournals on March 9, Copyright American Association for Cancer Research Author Manuscript Published OnlineFirst on March 0.  Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. as controls. As in the dose-finding studi survival data waspiled for bothbination studies to generate Kaplan-Meier survival curves. Letrozole Interim amines analysis on study day , as measured by mean tumor volumes for all surviving mi showed that treatment with L-BL 5 inbination with letrozole reduced mean tumor burden .

In the letrozole and L-BL 5bination study on day , when 5 of the untreated mice had Oleanolic Acid inhibitor reached maximum tumor burd mice in the CTX L-BL 5 and letrozole CTX L-BL 5 treatment groups showed a clear and significant survival advantage over untreated mic whereas the CTX placebo group was not significantly different from the untreated group . Letrozole inbination with L-BL 5 showed significantly improved survivalpared to letrozole alone . As per our desi we continued the study until 5 of mice in the CTX placebo group were euthanized due to excessive tumor burd at which time survival data was calculated again for this study . Confirming our previous findin animals in the CTX L-BL 5 and letrozole.