PAO1 again formed the unique BLS but failed to form any biofilm on the plastic surface (data not shown). In contrast, when we repeated the experiment with TSBDC, an iron-deficient medium in which P. aeruginosa grows planktonically and develops conventional biofilm, PAO1 formed a thick mature biofilm attached

to the coverslip surface (data not shown). Figure 1 P. aeruginosa PAO1 forms BLS within the ASM+. After 48 h of growth at 37°C under 20% EO2/static conditions, PAO1/pMRP9-1 developed BLS that were confined to the ASM+ and not attached to the surface of the microtiter plate. The composition of the ASM+ and the bacterial AP26113 purchase inoculation are described in Methods. The gelatinous mass containing the BLS was visualized in situ by CLSM. (A) CLSM micrograph Doramapimod supplier of the PAO1/pMRP9-1 BLS; magnification, 10X; bar, 200.00 nm. (B) 3-D image analysis revealing the architecture of the BLS shown in (A); box, 800.00 pixels (px) W x 600 px H; bar, 100 px. (C) CLSM micrograph of the well bottom after the removal of the gelatinous mass showing no attached bacteria or biofilm (the scattered fluorescence observed is due to autofluorescing debris). Table 1 Effect

of time and environmental variables on PAO1/pMRP9-1 BLS www.selleckchem.com/products/verubecestat.html Variable Image stacks (#) a Total biovolume (μm3/μm2) b Mean thickness (μm) c Roughness coefficient d Total surface area × 107(μm2) e Surface to volume ratio (μm2/μm3) f Time (under 20% EO 2 ) 48 h 10 6.52 ± 0.43 11.6 ± 0.28 0.53 ± 0.02 1.65 ± 0.24 1.54 ± 0.10 72 h 10 11.1 ± 0.40 15.5 ± 0.23 0.18 ± 0.02 2.15 ± 0.03 1.01 ± 0.04 6 d 10 18.2 ± 0.32 17.8 ± 0.06 0.02 ± 0.00 0.96 ± 0.12 0.28 ± 0.04 Mucin concentration (3 d under 20%

EO 2 ) 1X 10 11.1 ± 0.40 15.5 ± 0.23 0.18 ± 0.02 2.15 ± 0.03 1.01 ± 0.04 0.5X 10 13.5 ± 0.24 17.0 ± 0.05 0.08 ± 0.00 2.44 ± .045 0.94 ± 0.03 2X 10 15.4 ± 0.35 17.3 ± 0.08 0.06 ± 0.00 1.97 ± .098 0.67 ± 0.05 DNA concentration (3 ZD1839 d under 20% EO 2 ) 1X 10 11.1 ± 0.40 15.5 ± 0.23 0.18 ± 0.02 2.15 ± 0.03 1.01 ± 0.04 0.5X 10 2.42 ± 0.54 4.37 ± 1.37 1.33 ± 0.20 0.76 ± .220 1.55 ± 0.15 1.5X 10 2.48 ± 0.22 5.52 ± 0.64 1.07 ± 0.07 0.96 ± .086 2.02 ± 0.01 Oxygen concentration (EO 2 ) g 20% 10 11.1 ± 0.40 15.5 ± 0.23 0.18 ± 0.02 2.15 ± 0.03 1.01 ± 0.04 10% 10 19.4 ± 0.28 17.9 ± 0.04 0.01 ± 0.00 0.46 ± 0.12 0.13 ± 0.03 0% 10 0.28 ± 0.19 0.41 ± 0.27 1.94 ± 0.04 0.07 ± 0.06 1.75 ± 0.30 a Each experiment was done in duplicate.