21 C2C12 muscle cells treated with 5 mM leucine have demonstrated suppressed MAFbx/atrogin-1 and MuRF1 mRNA levels.22 Therefore, leucine supplementation

to older adults may serve as a potential strategy to combat the progression of sarcopenia. The dose–response of leucine supplementation is unknown, and future studies should focus on finding effective and safe doses for the use of leucine as an anti-atrophic agent in sarcopenia.18 In the meantime, older adults should be encouraged Inhibitors,research,lifescience,medical to consume a diet high in EAAs, in particular leucine-rich food sources such as beef, fish, and legumes.6 Vitamin D has AZD5363 in vitro recently received recognition as another potential intervention modality for sarcopenia.6 Recent findings have demonstrated that vitamin D plays an important role in skeletal muscle tissue by maintaining the function of type II fibers, preserving muscle strength and preventing falls.23 Vitamin D receptor knock-out mice are characterized by growth retardation, muscle impairment, and smaller diameters of muscle fiber than those of wild-type mice.24 Inhibitors,research,lifescience,medical Older adults are at increased risk of vitamin D insufficiency

Inhibitors,research,lifescience,medical due to various factors. As people age, the skin’s ability to synthesize vitamin D efficiently is reduced, and the kidney is less able to convert vitamin D to its active form; in addition, inadequate sunlight exposure which is essential for vitamin D synthesis and low consumption of dietary vitamin D are common among the elderly.24–26 Indeed, the prevalence of vitamin D insufficiency in the elderly has been estimated at 78%.26 Clinical, in-vivo, and in-vitro studies have shown that vitamin D affects muscle strength and function.26 For instance, Bischoff-Ferrari et al.27 have shown that higher concentrations Inhibitors,research,lifescience,medical of vitamin D are associated with better musculoskeletal function in the lower extremities than lower vitamin D concentrations in people over the age of 60. Also, Pfeifer et al.28 have

demonstrated that combined vitamin D (800 IU/day) and calcium (1,000 mg/day) supplementation are superior to calcium alone in reducing Inhibitors,research,lifescience,medical the number of falls and improving muscle function and strength in community-dwelling older individuals. On the cellular level, in-vitro studies have demonstrated that vitamin D can stimulate before proliferation and differentiation of myoblasts. Signaling pathways involved in vitamin D-associated proliferation and differentiation of myoblasts include the mitogen-activated protein kinases (MAPK) pathways such as the extracellular signal-regulated kinase 1 and 2 (ERK1/2), p38 MAPK, and c-Jun NH2-terminal 1 and 2 MAPK (JNK1/2).26 Nevertheless, the exact mechanisms of vitamin D action in skeletal muscle and how it promotes improvements in muscular performance are yet to be clear, and further studies are needed. The role of vitamin D in skeletal muscle and its ability to prevent muscular deterioration has been demonstrated at all research levels.