8-kb chromosomal region of Xanthomonas axonopodis pv. citri strain 306 carrying genes that encode type III effectors and helper proteins. The presence of pXap41 in all X. arboricola pv. pruni genotypes was confirmed for eight strains by plasmid profiling and for 35 X. arboricola pv. pruni isolates with a new plasmid multiplex PCR assay. This plasmid was not detected in any other X. arboricola find more pathovars (n=12), indicating the potential for the application of the pXap41 PCR method as a pathovar-level detection and identification tool. Xanthomonas arboricola

pv. pruni (Vauterin et al., 1995; syn. Xanthomonas campestris pv. pruni Smith) is a plant pathogenic gammaproteobacterium that causes Fulvestrant in vitro bacterial spot on a wide range of commercial, ornamental and forest Prunus species (Ritchie, 1995). Outbreaks can significantly reduce crop yield, and result in tree or orchard loss, particularly on peach, apricot, nectarine, plum and prune. Symptoms appear on leaves, fruits and branches, ranging from necrotic angular lesions on leaves or sunken lesions on fruits to cankers and dieback of branches. Control options are limited, with most commercial cultivars

generally considered susceptible and prophylactic copper compounds sprays constrained by the development of pathogen resistance and environmental concerns with residues (Ritchie, 1995, 1999). In most countries, X. arboricola pv. pruni is regulated as a quarantine pathogen (Anonymous, 2000), with substantial additional economic burdens that this status entails (e.g. phytosanitary inspection, monitoring, eradication and trade restrictions).

Moreover, there is a suggested increasing invasion risk of this pathogen due to climate change, expanding cultivation of host crops, trending towards high-quality, but susceptible varieties (Anonymous, 2009; Palacio-Bielsa et al., 2010; Pothier et al., 2010; Marchi et al., 2011). Despite its regulatory and economic significance, relatively little is known about the genetics of X. arboricola pv. pruni (or any other X. arboricola pathovar) compared with other Xanthomonas species, and this has for the most part been limited to biodiversity analysis (Zaccardelli et al., 1999; Boudon et al., 2005). Plasmids are known as influential factors in the pathogenesis and evolution of bacteria (Ziebuhr et al., 1999). Y-27632 2HCl Their ability to transfer between species is a way for bacteria to acquire new genes or target new hosts. Characterizing plasmids is an important step towards understanding the mechanisms of virulence and their evolution, and can impact the design of effective disease management strategies (Coplin, 1989). Plasmid sequences have been reported from Xanthomonas axonopodis pv. citri, X. campestris pv. vesicatoria, Xanthomonas albilineans and X. axonopodis pv. glycines (Weng et al., 1997; da Silva et al., 2002; Thieme et al., 2005; Kim et al., 2006; El Yacoubi et al., 2007; Pieretti et al.