, 2010). The economics of processing tropical crops could be improved by developing higher-value use for their by-products. It has now been reported that the by-products of tropical fruits contain high levels of various health enhancing substances that can be extracted to provide nutraceuticals (Gorinstein et al., 2011). In addition, the full utilization of fruits could lead the industry to a lower-waste agribusiness, increasing industrial profitability. The use of the entire plant tissue could have economic benefits to producers and a beneficial impact on the environment, leading to

a greater diversity of products (Peschel et al., 2006). A number of studies for determination of the bioactive composition of tropical fruits have been reported (Barreto et al., 2009, Pierson et al., buy PFI-2 2012, Rufino et al., 2010 and Sousa et al., 2012); however, a detailed comprehensive characterization including their by-products and individual phenolic compounds (resveratrol and coumarin) has not been reported so far. Furthermore, variations in sample preparation may also affect results greatly, yielding conflicting and non-comparable results, and this

is a problem deserving attention from researchers. Taking into account the potential of compounds present in pulps and by-products of tropical fruits as anti-inflammatory and antioxidant agents, and the fact that very few reports exist to date on the characterization of polyphenolic and carotene compounds in these products, this study aimed to quantify and compare the major Clomifene bioactive Akt inhibitor compounds found in pulp and by-products of commercialized tropical fruits from Brazil. Resveratrol, coumarin, gallic acid standards and solvents used for HPLC analysis (acetonitrile and methanol) were obtained from Sigma Aldrich (Steinheim, Germany). All other reagents were analytical grade and were purchased from VWR International (Radnor, PA). Samples consisted

of fresh, non-pasteurized frozen pulps of pineapple (Ananas comosus L.), acerola (Malpighia emarginata D.C.), monbin (Spondias mombin L.), cashew apple (Anacardium occidentale L.), guava (Psidium guajava L.), sourspop (Annona muricata L.), papaya (Carica papaya L.), mango (Mangifera indica L.), passion fruit (Passiflora edulis Sims), surinam cherry (Eugenia uniflora L.), sapodilla (Manikara zapota L.) and tamarind (Tamarindo indica L.) were obtained from fruit processing plants in the state of Ceará, Brazil. The by-products were used from the production process of pulps, obtained after pulping of: pineapple (peel and pulp’s leftovers), acerola (seed), cashew apple (peel and pulp’s leftovers), guava (peel, pulp’s leftovers, and seed), soursop (pulp’s leftovers and seed), papaya (peel, pulp’s leftovers, and seed), mango (peel and pulp’s leftovers), passion fruit (seed), surinam cherry (pulp’s leftovers), and sapodilla (peel, pulp’s leftovers and seed).