[16]. This questionnaire has been translated

into various languages and undergone cultural adaptation and validation. The Spanish questionnaire is version 2.1 of the original one [17] and consists of 35 items grouped into 11 domains: General Health Perceptions, Pain, Physical Functioning, Role Functioning, Social Functioning, Mental Health, Energy, Health Distress, Cognitive Functioning, Overall Quality of Life and Health Transition. In addition to these subscales, the Physical Health summary score (PHS) and Mental Health summary score (MHS) can be calculated by standardizing the score Dabrafenib nmr of each domain using weighting coefficients given by the authors of the questionnaire [18]. The MOS-HIV domains are scored as summated rating scales from 0 (worst state of health possible) to 100 (best state of health possible). The internal consistency of the scales is high (Cronbach’s α=0.78–0.89) and the Smad inhibitor test–retest reliabilities of the Physical and Mental Health indexes are 0.58 and 0.85, respectively

[18]. To evaluate which variables may be predictors of HRQL, a specific questionnaire was created in which the second person was used as a formal manner of address (in Spanish: the form usted) in order to avoid possible discrepancies between the questions made and the patient’s subjective feelings. Data collected included the following. Sociodemographic variables: age, sex, nationality, marital status, domestic situation, parenthood, educational background, employment status, income level, sexual orientation (heterosexual, homosexual

or bisexual), and tobacco, alcohol and drug use. Clinical variables: CD4 cell count [determined by flow cytometry using FACSCalibur (Becton-Dickinson, Franklin Lakes, New Jersey, USA)], viral load [determined by polymerase chain reaction (PCR) using the Ultrasensitive Cobas Amplicor HIV Monitor (Roche, Pleasanton, very California, USA)], HIV transmission group, AIDS classification [Centers for Disease Control and Prevention (CDC) criteria], symptoms (list compiled from contributions in the literature revised and from our observations in clinical practice) and comorbidity [dyslipidaemia, hypertension, diabetes mellitus, chronic hepatitis C virus (HCV) infection and chronic bronchopathy]. Variables related to antiretroviral therapy (ART): adherence, type of regimen and its administration, and number of pills prescribed per day. Psychological variables: presence of symptoms of depression, health care satisfaction level, degree of trust in the attending clinical staff and self-perception of the level of support received. ART adherence was evaluated using the Simplified Medication Adherence Questionnaire (SMAQ) created by the Spanish group Grupo Español para el Estudio Multifactorial de la Adherencia (GEEMA) [19], which has been shown to have 72% sensitivity and 91% specificity.