1. A high magnification of the PE/TiO2 NLC (Figure 3b) shows that the interface between the PE and TiO2 layers is not sharp completely, but somewhat diffuse, indicating a sizeable interpenetration between the TiO2 and organic PE components [10]. A selected-area electron diffraction pattern taken from the dotted-circle region in Figure 3a was presented in the inset of Figure 3b, revealing the diffuse diffraction ring corresponding to the amorphous PE layers, while some diffraction spots exhibit the existence of crystallites. JAK inhibitor A high-resolution transmission electron microscopy (HRTEM)

image (Figure 3c) shows that some nanocrystallines (NCs) with different orientations have formed in the TiO2 layer and their sizes are in a range of about 5 to 15 nm. The

NC TiO2 might form during the CBD process rather than the TEM electron-beam irradiation since the TEM accelerating voltage we used was 200 keV rather than 400 keV [10]. The formation of the NC TiO2 might be related to the very thin TiO2 layers (approximately 17.9 nm) deposited in a short time (2 h) of the CBD process. In addition, the rough and thin PE layers assembled by few numbers of cycles (3 cycles) for the PAH/PSS might also play an important role in the heterogeneous nucleation of the TiO2 nanocrystallines. Figure 3 TEM cross-sectional images of the composite and HRTEM image of the interface. TEM cross-sectional images of the (PE/TiO2)4 nanolayered composite at (a) low magnification and (b) high magnification. (c) HRTEM image of inorganic TiO2 layer and organic/inorganic interface. Mechanical performance Figure 4a shows a typical

FG-4592 clinical trial load-indentation depth curve of the (PE/TiO2)4 NLC. In the loading stage, no pop-in behavior was detected, indicating that the NLC can be deformed continuously to the indentation depth of about 30 nm. In the unloading stage, the initially linear unloading reveals an elastic recovery. With a further unloading, the nonlinear variation of the load with the displacement reveals the non-elastic recovery, leading to a residual indentation depth of about 22 nm. Young’s modulus of the NLC determined from the contact area and the elastic contact stiffness [16] is 17.56 ± 1.35 GPa, which is much lower than that of the nacre (E = 50 GPa) [18]. Such a low Young’s ZD1839 manufacturer modulus may be attributed to the large volume fraction of organic PE layers due to R t ≈ 1.1. Based on the rule of mixture, Young’s modulus is estimated to be about 16.74 GPa by using = 27.5 GPa and E PE = 5 GPa [11], and this is close to the experimental result of the (PE/TiO2)4 NLC (17.56 GPa). The mean hardness of the (PE/TiO2)4 NLC determined by nanoindentation is 0.73 GPa with a standard deviation of 0.09 GPa. Using a general relation between hardness (H) and strength (σ) found in a lot of materials, , the mean strength of the NLC was calculated as about 245 MPa, which is quite close to the strength of shells reported in the literature (100 to 300 MPa) [10, 18]. Although R t ≈ 1.