002), duration of mechanical ventilation (P < 0.012) and intensive care unit stay (P < 0.005). A smaller prospective cohort study reported significantly raised PaO2 (P =
0.0091) and reduced PaCO2 (P < 0.0001) levels in the non-smokers as well as improved FVC and FEV1 (P < 0.0001). There were also reductions in duration of intubation (P < 0.0001), intensive care unit stay (P < 0.0001) and hospital stay (P < 0.0013). Another small cohort study reporting outcomes of heart transplantation demonstrated significant improvement in non-smokers in terms of survival (P = 0.031), duration of intubation (P = 0.05) and intensive care unit stay (P= 0.021). We conclude that there is strong evidence demonstrating superior outcomes in non-smokers following YM155 nmr cardiac surgery and advocate the necessity of smoking cessation as soon as possible prior to cardiac surgery.”
“An in vitro culture of Artemisia absinthium L. was established for production of commercially important secondary metabolites. Callus cultures were obtained by inoculating leaf explants on Murashige and Skoog (MS) medium supplemented with Thidiazuron (TDZ; SNX-5422 0.5-5.0 mgl(-1)) alone or in combination with either alpha-naphthalene acetic acid (NAA; 1.0 mgl(-1)) or Indole acetic acid (IAA; 1.0 mgl(-1)). The callus obtained in response to 1.0 mgl(-1) TDZ
and 1.0 mgl(-1) NAA was subcultured on the same medium to investigate its biomass accumulation and secondary metabolites production on weekly basis for 7 weeks. For submerged cultivation, 35 day old calli were cultured on MS basal media supplemented with 1.0 mgl(-1)
TDZ and 1.0 mgl(-1) NAA. Growth kinetics and secondary metabolites production were investigated in 3 day old suspension cultures for 42 days. Additionally, high performance liquid chromatography A1155463 (HPLC) based quantification of gallic acid, caffeic acid and catechin was carried out in cell suspension cultures. Seed germinated plantlets were used as control. Maximum levels of total phenolic content 3.57 mg GAE/g DW (control: 2.75 mg GAE/g DW), total flavonoid content 1.89 mg QE/g OW (control: 1.20 mg QE/g DW), and antioxidant activity 82.7% (control: 72.3%) were displayed by suspension cultures. Among the phenolic compounds, maximum level of gallic acid 104 mu gg(-1) (control: 21.3 mu gg(-1)), caffeic acid 27.40 mu gg(-1) (control: 28.5 mu gg(-1)) and catechin 92.0 mu gg(-1) (control: 68.10 mu gg(-1)) were detected in suspension cultures. The results indicate that cell suspension cultures of A. absinthium L have the potential for enhanced production of phenolics and, hence, highest antioxidant activity than callus culture and seed derived plantlets. (C) 2013 Elsevier B.V. All rights reserved.