The same tendency of IFN-γ mRNA, TNF-α mRNA, IL-17 mRNA and IL-6 mRNA were detected in the DSS and DSS+VD group. Conclusion: From the results obtained, spleen immunity was relieved in the chronic experimental colitis mice affected by 1,25 (OH)2D3, whose mechanism may be inhibiting the activation of Th1 and Th17 effectors. Key Word(s): 1.1,25 AZD0530 in vitro (OH)2D3; 2. Th1/Th17 cells; 3. immune response; 4. ulcerative colitis; Presenting Author: ROBERTA PICA Additional Authors: CLAUDIO CASSIERI, AURORA DE CAROLIS, ELEONORAVERONICA AVALLONE, MADDALENA ZIPPI, CLAUDIA CORRADO, ENRICO STEFANO, PIERO VERNIA, PAOLO PAOLUZI Corresponding

Author: ROBERTA PICA Affiliations: IG-IBD Objective: Inflammatory bowel diasease (IBD) patients are CS-dependent in 17–36% and refractory in 12–20%. Aim of the study has been to investigate the prevalence of CS dependence or resistance

in a single centre MK0683 ic50 series of Italian IBD patients, as well as the treatment options as CS-sparing agents in ulcerative colitis (UC) and Cronh’s disease (CD). Methods: Computerized data of consecutive IBD patients referred to our Centre, from 1990 to 2010, were studied. Results: One thousand three hundred and twenty-six patients were studied, 729 (55%) were male and 597 (45%) female. Of this 781 (58.9%) were affected by UC and 545 (41.1%) by CD. Three hundred and thirty-three (25.1%) patients were CS dependent (164 UC vs 169 CD, 21% and 31% respectively, p < 0.0001); 38 (2.9%) patients were CS-resistant (19 UC vs 19 CD, 2.4% and 3.5% respectively). Of this 63 patients with a follow-up < 12 months were excluded. Three hundred and eight patients (146 UC, 162 CD) were evaluated for treatment options as CS-sparing agents. Azathioprine was used in 191 patients (85 UC vs 106 CD), 32 underwent surgery (6 UC vs 26 CD, p = 0.0006), 16 were treated

with anti TNF-α agents (8 UC vs 8 CD), 13 with Cyclosporine (2 UC vs 11 CD, p = 0.0220), 4 with Methotrexate (3 UC vs 1 CD), 9 UC patients were treated with leukocytapheresis. Forty-three patients (33 UC vs 10 CD, p < 0.0001) refused other therapeutic options and continued on CS. Conclusion: The prevalence of CS-dependence was significantly higher in CD than in UC. Cyclosporine and surgery were significantly used in CD than UC. Key Word(s): 1. STEROID DEPENDENCE; 2. STEROID RESISTANCE; 3. IBD; Presenting Author: ROBERTA PICA Additional Aspartate Authors: MADDALENA ZIPPI, CLAUDIA CORRADO, ELEONORAVERONICA AVALLONE, CLAUDIO CASSIERI, AURORA DE CAROLIS, PAOLO PAOLUZI, PIERO VERNIA Corresponding Author: ROBERTA PICA Affiliations: IG-IBD Objective: Ulcerative colitis (UC) and Crohn’s disease (CD) may be associated with extraintestinal manifestations (EIMs). Aim of this retrospective study has been to investigate the prevalence, type and time of onset of EIMs in a large series of Italian IBD patients. Methods: 811 IBD patients regularly followed-up were studied. Mann-Whitney test and Fischer Exact test were used.