Cortical layer (22–)28–58(–87) μm (n = 30) thick, a hyaline to pale yellowish t. globulosa-angularis of thin-walled, globose, angular or oblong cells (4–)7–15(–20) × (3.5–)6–11(–15) μm (n = 60) in face view Cell Cycle inhibitor and in vertical section, penetrated by some hyphae of subcortical origin. Hairs on mature stroma (10–)16–30(–40) × (3.5–)5–9(–11) μm (n = 20), inconspicuous, 1–3 celled, smooth or slightly verruculose, of variable shape, often ampulliform or appearing as short, submoniliform chains of cells. Subcortical tissue a hyaline t. intricata of thin-walled hyphae (2–)4–7(–9) μm (n = 30) wide, in part appearing

as t. globulosa due to variable orientation of hyphae. Subperithecial tissue www.selleckchem.com/products/ml323.html a dense t. epidermoidea of thin-walled cells (4.5–)6–22(–34) × (3.5–)6–13(–16) μm (n = 30), penetrated by hyphae (4–)5–10(–12) μm (n = 20) wide. Stroma base a loose hyaline t. intricata of thin-walled hyphae (3–)4–9(–12) μm (n = 20) wide. Asci (84–)93–115(–124) × (5.0–)5.3–6.3(–7.0) μm, stipe (2–)5–17(–30) μm long (n = 30); base not or slightly thickened, no croziers seen. Ascospores hyaline, verrucose or spinulose, cells dimorphic, distal cell (3.7–)4.4–5.2(–5.8) × (3.5–)3.8–4.4(–4.8)

μm, l/w (1.0–)1.1–1.3(–1.5) (n = 90), subglobose, oval or oblong, proximal cell (3.6–)4.7–6.0(–7.0) × (2.8–)3.3–4.0(–4.3) μm, l/w (1.0–)1.3–1.7(–2.1) (n = 90), oblong or wedge-shaped; often turning yellow or orange after ejection. Cultures and anamorph: optimal growth at 25°C on CMD and PDA, at 25–30°C on SNA, ATM/ATR phosphorylation faster on PDA than on CMD and SNA;

no growth at 35°C. On CMD after 72 h 12–14 mm at 15°C, 42–49 mm at 25°C, 33–36 mm at 30°C; mycelium covering the plate after 4–5 days at 25°C. Colony hyaline, hardly visible, thin, smooth, not zonate; hyphae loosely disposed, thick, sinuous, sometimes with submoniliform Dynein thickenings. Aerial hyphae sparse and short, longer towards the distal margin. No autolytic activity and coilings noted. No distinct odour, no pigment noted, but agar sometimes becoming slightly reddish brown after prolonged storage. Chlamydospores noted after 8–10 days, infrequent. Conidiation noted after 4–6 days, effuse, sparse, mostly in the distal half of the plate, macroscopically invisible, colourless. Conidiophores simple, verticillium-like, phialides subulate, conidial heads wet, large. At 15°C concentric zones of unequal width noted, conidiation after 2–3 weeks sometimes in compact white pustules, with phialides in part converging gliocladium-like. Chlamydospores abundant, noted after 4–5 days. At 30°C slight diffuse greenish-yellowish pigment noted in the agar after 1 weeks. Conidiation noted after 2–3 days, more abundant than at 25°C, mainly along the margin; conidiophores acremonium- and verticillium-like, phialides subulate, in whorls of 2–3.