2 2. Conidia ellipsoid, (14–)16–19(–22) × (6–)7–9(–11) µm, ratio 2.1:1 (l:w) ………………………… Ps. eucalypti 2. Conidia variable in shape, subglobose to bean-shaped, (6.5–)15.5–17(–19) × (6.5–)7.5–9(–10.5) µm, ratio 2:1 (l:w) …………………………………….. Ps. variabile *Sporulating ISRIB research buy on MEA in culture. Discussion Results of this study have elucidated considerable confusion that has surrounded the taxonomy of one of the fungal pathogens most commonly encountered on leaves of Eucalyptus in plantations globally. Phylogenetic inference of DNA sequence data thus showed that the fungus known as Cryptosporiopsis eucalypti and encountered in many treatments of Eucalyptus diseases (Sharma 1994; Sankaran et al. 1995; Old et
al. 2002, 2003) is the anamorph of a member of the Diaporthales (99% bootstrap support), and not the Dermateaceae (Helotiales) along with Cryptosporiopsis s. str. The Oligomycin A purchase Eucalyptus pathogen that has been treated as C. eucalypti since 1995 has thus been placed in a novel genus
as Pseudoplagiostroma eucalypti. This study includes 39 isolates collected from Eucalyptus in plantations on four continents and from 10 countries. The combined sequence data sets for this collection of isolates delineate three distinct species within a monophyletic lineage. The major clade (P. eucalypti) includes 27 isolates, while the second clade (P. oldii) includes two isolates (CBS 124808 and CBS 115722) and the third clade (P. variabile) consists of a single isolate, CBS 113067. The monophyly of Pseudoplagiostoma is strongly supported by morphological characteristics. While all three species are very similar on OA, PDA, and PNA, they can easily be distinguished in culture on MEA. The conidial wall of Ps. oldii turns brown at maturity, suggesting that this
feature can be used to distinguish them (also on PNA and OA, but not on PDA). Colonies of Ps. variabile grow more slowly than those of Ps. eucalypti and Ps. oldii. It produces fewer conidia on MEA, undergoes microcyclic conidiation, and its conidia are not uniform, ranging learn more from subglobose to ellipsoid. These features should make this widely distributed group of fungi easy to identify in Eucalyptus disease surveys. Within the Diaporthales, Pseudoplagiostoma is more similar to members of the Gnomoniaceae based on the morphological characters of its teleomorph, such as solitary, thin-walled, immersed ascomata with lateral beaks lacking stromata, asci with a distinct ring, and medianly 1-septate ascospores less than 25 mm long (Monod 1983; Barr 1978; Samuels and Blackwell 2001; Castlebury et al. 2002; Sogonov et al. 2008). In 3-Methyladenine purchase contrast, in the Valsaceae and Sydowiellaceae, stromatic and non-stromatic tissues are present (Wehmeyer 1975; Rossman et al. 2007). Also, in other families of Diaporthales such as Cryphonectriaceae, Diaporthaceae, Melanconidaceae and Pseudovalsaceae, the stromatic tissues are often well-developed (Castlebury et al. 2002; Gryzenhout et al. 2006; Voglmayr and Jaklitsch 2008).